Whole Transcriptome Analysis: Implication to Estrous Cycle Regulation

Regulation of the estrous cycle by neutrophils via opioid peptides.

We found previously that neutrophil-depleted mice exhibited significant blockading of both the regular estrous cycle and cyclic changes of steroid hormone levels. In this study, we aimed at elucidation of the underlying mechanism. To examine the possibility that an increase in bacteria in the vaginal vault of neutrophil-depleted mice causes blockading of the estrous cycle, we treated neutrophil...

Neutrophils via Opioid Peptides Regulation of the Estrous Cycle by

Estrous Cycle Synchronization In

E STRUS and ovulation in gilts have been successfully inhibited by daily injections of progesterone (Ulberg et al., 1951; Baker et al., 1954) and more recently by the inclusion of progestational compounds in the feed (Nellor, 1960; Dzuik and Baker, 1962). The successful use of orally active progestational agents to synchronize the estrous cycles of gilts without a subsequent reduction in fertil...

The rat estrous cycle revisited: a quantitative and qualitative analysis

The rat has been elected as the main animal model in several studies involving reproduction. However, there are scarce and conflicting data related to its estrous cycle. It comprises phases characterized by different cell types in vaginal smears (proestrus, estrus, metestrus and diestrus). Nevertheless, this classification and the time span of each phase are controversial and may induce mislead...

Whole-transcriptome microarray analysis reveals regulation of Rab4 by RBM5 in neurons

RNA binding motif 5 (RBM5) is a nuclear protein that modulates gene transcription and mRNA splicing in cancer cells. The brain is among the highest RBM5-expressing organ in the body but its mRNA target(s) or functions in the CNS have not been elucidated. Here we knocked down (KO) RBM5 in primary rat cortical neurons and analyzed total RNA extracts by gene microarray vs. neurons transduced with ...